Defective bacteriophage lambda chromosome, potential vector for DNA fragments obtained after cleavage by bacillus amyloliquefaciens endonuclease (BamI)
نویسندگان
چکیده
منابع مشابه
Characterization of bacteriophage lambda excisionase mutants defective in DNA binding.
The bacteriophage lambda excisionase (Xis) is a sequence-specific DNA binding protein required for excisive recombination. Xis binds cooperatively to two DNA sites arranged as direct repeats on the phage DNA. Efficient excision is achieved through a cooperative interaction between Xis and the host-encoded factor for inversion stimulation as well as a cooperative interaction between Xis and inte...
متن کاملRestriction endonuclease Hin dIII cleavage site map of bacteriophage P22.
The 14 Hind111 cleavage sites on P22 DNA have been mapped. Hind111 cleavage sites were located relative to EcoRI sites by determining the molecular weights and map order of fragments produced by HindIII, or Hind111 and EcoRI digestion. Molecular weights were estimated from the electrophoretic mobility of fragments. The Hind111 fragment order was established by Hind111 cleavage of segments of th...
متن کاملMolecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI.
The highly specific restriction endonucleases Sa1I and BamI produce DNA fragments with complementary, cohesive termini that can be covalently joined by DNA ligase. The Escherichia coli kanamycin resistance factor pML21 has one SalI site, at which DNA can be inserted without interfering with the expression of drug resistance or replication of the plasmid. A more convenient cloning vehicle can be...
متن کاملEcoRI restriction endonuclease cleavage site map of bacteriophage P22DNA.
‘l’l~e SWYW EcoKI restriction endonuclease cleavage sites in bacteriophage P%d DNA have been mapped. The cleavage site map of circularly permut.ed P22 linear DNA is a circle. The positions of EcoRI sites in the early region of the P22 genomc were determined by comparing products of EcoRI digestion of maturtx liaear P22 chromosomes with the EcoRI cleavage fragments of DNA of three XimmP22 hybrid...
متن کاملGenetic mapping of a defective bacteriophage on the chromosome of Bacillus subtilis 168.
A genetic marker responsible for the killing activity of PBSX, a defective phage carried by Bacillus subtilis 168, has been located on the bacterial chromosome. Two mutant strains of B. subtilis 168, which produced tailless phage particles upon mitomycin C induction, were shown to carry lesions, designated xtl-1 and xtl-2, which were linked by transformation and PBS1-mediated transduction to me...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1975
ISSN: 0014-5793
DOI: 10.1016/0014-5793(75)80099-8